Microbiological control of milk and dairy products
Microbiological control of pasteurized and ultra-pasteurized milk (PAST).
The test tube contains a mixture of fluorogenic substrates that participate in microbial metabolism, producing fluorescent products. The increase in the concentration of fluorescent products per unit of time is proportional to the biomass of living microorganisms.
The BioTF turbidofluorimeter can analyze up to30 samples per hour. The total time for quantitative microbiological testing of pasteurized and ultra-pasteurized milk does not exceed 60 minutes. This method allows for the detection of single microbial cells that often do not grow on agar nutrient media but cause product spoilage.
Fill two test tubes with 1.25 cm³ of milk.
Add 2 drops of sterilizing agent to the labeled test tube.
The contents of the test tubes are mixed for 10 seconds using a vortex.
Test tubes are placed in an incubator at 37°C for 60 minutes.
The contents of the test tubes are mixed for 10 seconds using a vortex.
The test tube with the label is placed in the BioTF turbidofluorimeter
Select the PAST application and press BLANK<M0> to record the control value
The second test tube is placed in the BioTF turbidofluorimeter
Select the PAST application and determine the total number of microorganisms