Heat Treatment Efficiency Test
Rapid evaluation of milk heat treatment efficiency based alkaline phosphatase (ALP) activity as an indicator of proper milk heat treatment. ALP activity is measured by kinetic method in biochemical reaction of hydrolysis of phosphate which is present in non-fluorescent substrate by ALP. As a result of hydrolysis non-fluorescent substrate transforms into its fluorescent form. The specific rate of fluorescence is proportional to ALP activity. ALP application registers the dynamics of fluorescence, calculates numerical value of ALP activity and estimates the efficiency of heat treatment process.
1. Heat a microtube with 0.9 ml of Ringer's solution to a temperature of 37 C
2. Add 0.1 ml of milk to a microtube with 0.9 ml of Ringer's solution and mix thoroughly.
3. Pour the contents of the microtube into the activated “ALP” test tube.
4. Vortex for 10 seconds.
5. The test tube is placed in the BioTF turbidofluorimeter.
6. Select the ALP application and press the green button.